Hotspot

ndhH-rps15

SSC · 120140-120246 · 107 bp

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Pi 0.402

MarkerSeek score 77.10

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 107
variable_sites: 41
pi: 0.402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	CATTGGTCCTGATAAGCCCC	TATCGCAAAGGGGTCTACGA	55.0	50.0	58.0	58.2	907 bp	0.5	58.0
ndhH-rps15_p2	CCTCTCCACCAATAATGCCC	TATCGCAAAGGGGTCTACGA	55.0	50.0	58.0	58.2	875 bp	0.5	58.0
ndhH-rps15_p3	TTTCCAATCGTTCTGGTGCA	TATCGCAAAGGGGTCTACGA	45.0	50.0	58.0	58.2	509 bp	0.5	57.9
ndhH-rps15_p4	GCCATAAAAGGACCCAACCA	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.1	58.2	606 bp	0.5	57.6
ndhH-rps15_p5	TGCCATAAAAGGACCCAACC	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.1	58.2	607 bp	0.5	57.6