Hotspot

ndhG-ndhI

SSC · 115909-116149 · 241 bp

Back to Gladiolus

Pi 0.5

MarkerSeek score 76.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 241
variable_sites: 112
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AGTAATACTACCCCCAGCCC	TACATCAGAGCGTTTCCGTG	55.0	50.0	57.9	58.0	724 bp	0.5	59.0
ndhG-ndhI_p2	GTAATACTACCCCCAGCCCT	TACATCAGAGCGTTTCCGTG	55.0	50.0	57.9	58.0	723 bp	0.5	59.0
ndhG-ndhI_p3	AGTAATACTACCCCCAGCCC	AACAATACGAGCTGCACGAT	55.0	45.0	57.9	58.0	826 bp	0.5	59.0
ndhG-ndhI_p4	GTAATACTACCCCCAGCCCT	AACAATACGAGCTGCACGAT	55.0	45.0	57.9	58.0	825 bp	0.5	59.0
ndhG-ndhI_p5	GTAATACTACCCCCAGCCCT	TGTTCCCTATGGTGACTGGT	55.0	50.0	57.9	58.0	865 bp	0.5	58.8