Hotspot

ndhF-rpl32

SSC · 109839-110369 · 531 bp

Back to Gladiolus

Pi 0.415

MarkerSeek score 81.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 531
variable_sites: 201
pi: 0.415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAATTGTGCCGATTTCGCAA	ATTGGAAGTCGAGCCATGAC	45.0	50.0	58.0	58.0	1475 bp	0.5	59.6
ndhF-rpl32_p2	GAATTGTGCCGATTTCGCAA	GGAAGTCGAGCCATGACAAT	45.0	50.0	58.0	58.0	1472 bp	0.5	59.6
ndhF-rpl32_p3	GGCGGAAAACACCATAGCTA	ATTGGAAGTCGAGCCATGAC	50.0	50.0	58.0	58.0	911 bp	0.5	59.5
ndhF-rpl32_p4	GGCGGAAAACACCATAGCTA	GGAAGTCGAGCCATGACAAT	50.0	50.0	58.0	58.0	908 bp	0.5	59.5
ndhF-rpl32_p5	CTGCAATTGGTCGTGTGAAC	GGAAGTCGAGCCATGACAAT	50.0	50.0	58.0	58.0	1254 bp	0.5	59.5