primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ndhF	1	AGAATGCCGTCGCCTATTTT	GATGCATGCTACGATCCGTA	20	20	45.0	50.0	58.0	57.7	0.0	20.3	0.386084	107622	109838	2838-2838	0.500000	56.9
ndhF_p2	ndhF	2	GCCGTCGCCTATTTTCACTA	GATGCATGCTACGATCCGTA	20	20	50.0	50.0	58.1	57.7	0.0	20.3	0.417130	107622	109838	2833-2833	0.500000	56.7
ndhF_p3	ndhF	3	AGAATGCCGTCGCCTATTTT	TTGATGCATGCTACGATCCG	20	20	45.0	50.0	58.0	58.5	0.0	20.3	0.537218	107622	109838	2840-2840	0.500000	55.7
ndhF_p4	ndhF	4	GCCGTCGCCTATTTTCACTA	TTGATGCATGCTACGATCCG	20	20	50.0	50.0	58.1	58.5	0.0	20.3	0.568264	107622	109838	2835-2835	0.500000	55.5
ndhF_p5	ndhF	5	AATGCCGTCGCCTATTTTCA	GATGCATGCTACGATCCGTA	20	20	45.0	50.0	58.3	57.7	0.0	20.3	0.602465	107622	109838	2836-2836	0.500000	55.2
ndhF-rpl32_p1	ndhF-rpl32	1	GAATTGTGCCGATTTCGCAA	ATTGGAAGTCGAGCCATGAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054002	109839	110369	1475-1475	0.500000	59.6
ndhF-rpl32_p2	ndhF-rpl32	2	GAATTGTGCCGATTTCGCAA	GGAAGTCGAGCCATGACAAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054002	109839	110369	1472-1472	0.500000	59.6
ndhF-rpl32_p3	ndhF-rpl32	3	GGCGGAAAACACCATAGCTA	ATTGGAAGTCGAGCCATGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062519	109839	110369	911-911	0.500000	59.5
ndhF-rpl32_p4	ndhF-rpl32	4	GGCGGAAAACACCATAGCTA	GGAAGTCGAGCCATGACAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062519	109839	110369	908-908	0.500000	59.5
ndhF-rpl32_p5	ndhF-rpl32	5	CTGCAATTGGTCGTGTGAAC	GGAAGTCGAGCCATGACAAT	20	20	50.0	50.0	58.0	58.0	7.7	0.0	0.066395	109839	110369	1254-1254	0.500000	59.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TACGGATCGTAGCATGCATC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.7	58.0	22.0	0.0	0.355528	110544	111332	1119-1119	0.500000	57.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TACGGATCGTAGCATGCATC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.7	58.0	22.0	0.0	0.356898	110544	111332	1120-1120	0.500000	57.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TACGGATCGTAGCATGCATC	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	57.7	57.9	22.0	0.0	0.416531	110544	111332	1597-1597	0.500000	56.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TACGGATCGTAGCATGCATC	TCGAAGAAATCCAACGGGTC	20	20	50.0	50.0	57.7	57.9	22.0	0.0	0.436647	110544	111332	1447-1447	0.500000	56.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TACGGATCGTAGCATGCATC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.7	57.9	22.0	0.0	0.471974	110544	111332	1129-1129	0.500000	56.2
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	CTCAAAAACTCGCACGAACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026547	111500	112456	1183-1183	0.500000	59.8
ccsA_p2	ccsA	2	GTGAAATCGGTAGACACGCT	GGTTCTCAAAAACTCGCACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026547	111500	112456	1187-1187	0.500000	59.8
ccsA_p3	ccsA	3	TGAAATCGGTAGACACGCTG	CTCAAAAACTCGCACGAACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027918	111500	112456	1182-1182	0.500000	59.8
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	GGTTCTCAAAAACTCGCACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027918	111500	112456	1186-1186	0.500000	59.8
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	TGCATTACCGGGAATGAGTG	20	20	50.0	50.0	58.0	58.0	0.0	38.4	0.044788	111500	112456	1691-1691	0.500000	59.6
ndhD_p1	ndhD	1	GAGGCATGGGGGTCTTATTG	AAACAACTCGAAGCATGGGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.031861	112687	114192	2121-2121	0.500000	59.7
ndhD_p2	ndhD	2	CGTGCGAGTTTTTGAGAACC	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.037461	112687	114192	1838-1838	0.500000	59.7
ndhD_p3	ndhD	3	GGTTCGTGCGAGTTTTTGAG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.037461	112687	114192	1842-1842	0.500000	59.7
ndhD_p4	ndhD	4	GAGGCATGGGGGTCTTATTG	GGCATGAAACAACTCGAAGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.044014	112687	114192	2127-2127	0.500000	59.6
ndhD_p5	ndhD	5	CGTGCGAGTTTTTGAGAACC	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049614	112687	114192	1844-1844	0.500000	59.6
psaC_p1	psaC	1	GATTGACCAAAAAGCCCGTG	ATCACAAGCCGAAACATGGT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.154873	114315	114560	938-938	0.500000	58.8
psaC_p2	psaC	2	CCCCCTGCAGTGTACAAAAT	ATCACAAGCCGAAACATGGT	20	20	50.0	45.0	58.1	58.1	13.8	0.0	0.166944	114315	114560	1476-1476	0.500000	58.7
psaC_p3	psaC	3	GATTGACCAAAAAGCCCGTG	GCGATTGGGCTAGCTATTGT	20	20	50.0	50.0	57.9	58.1	0.0	15.7	0.180338	114315	114560	779-779	0.500000	58.6
psaC_p4	psaC	4	GATTGACCAAAAAGCCCGTG	AGTCCCTTGGTCCTTTCTCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.182977	114315	114560	521-521	0.500000	58.5
psaC_p5	psaC	5	CCCCCTGCAGTGTACAAAAT	GCGATTGGGCTAGCTATTGT	20	20	50.0	50.0	58.1	58.1	13.8	15.7	0.192409	114315	114560	1317-1317	0.500000	58.5
ndhE_p1	ndhE	1	ACCCATGCTTCGAGTTGTTT	AGGGCTGGGGGTAGTATTAC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.132551	114902	115207	1517-1517	0.500000	58.9
ndhE_p2	ndhE	2	ACCCATGCTTCGAGTTGTTT	GGGCTGGGGGTAGTATTACT	20	20	45.0	55.0	58.0	57.9	0.0	1.3	0.132551	114902	115207	1516-1516	0.500000	58.9
ndhE_p3	ndhE	3	GCTTCGAGTTGTTTCATGCC	AGGGCTGGGGGTAGTATTAC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.144704	114902	115207	1511-1511	0.500000	58.8
ndhE_p4	ndhE	4	GCTTCGAGTTGTTTCATGCC	GGGCTGGGGGTAGTATTACT	20	20	50.0	55.0	58.0	57.9	0.0	1.3	0.144704	114902	115207	1510-1510	0.500000	58.8
ndhE_p5	ndhE	5	GATTGACCAAAAAGCCCGTG	GGGCTGGGGGTAGTATTACT	20	20	50.0	55.0	57.9	57.9	0.0	1.3	0.178380	114902	115207	1636-1636	0.500000	58.6
ndhG_p1	ndhG	1	ACCATGTTTCGGCTTGTGAT	TACATCAGAGCGTTTCCGTG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.099501	115378	115908	1422-1422	0.500000	59.2
ndhG_p2	ndhG	2	ACCATGTTTCGGCTTGTGAT	AACAATACGAGCTGCACGAT	20	20	45.0	45.0	58.1	58.0	0.0	25.3	0.100445	115378	115908	1524-1524	0.500000	59.2
ndhG_p3	ndhG	3	ACAATAGCTAGCCCAATCGC	TACATCAGAGCGTTTCCGTG	20	20	50.0	50.0	58.1	58.0	12.1	0.0	0.124966	115378	115908	1581-1581	0.500000	59.0
ndhG_p4	ndhG	4	ACAATAGCTAGCCCAATCGC	AACAATACGAGCTGCACGAT	20	20	50.0	45.0	58.1	58.0	12.1	25.3	0.125910	115378	115908	1683-1683	0.500000	59.0
ndhG_p5	ndhG	5	AATAGCTAGCCCAATCGCTG	TACATCAGAGCGTTTCCGTG	20	20	50.0	50.0	57.8	58.0	12.1	0.0	0.185078	115378	115908	1579-1579	0.500000	58.5
ndhG-ndhI_p1	ndhG-ndhI	1	AGTAATACTACCCCCAGCCC	TACATCAGAGCGTTTCCGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.123008	115909	116149	724-724	0.500000	59.0
ndhG-ndhI_p2	ndhG-ndhI	2	GTAATACTACCCCCAGCCCT	TACATCAGAGCGTTTCCGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.123008	115909	116149	723-723	0.500000	59.0
ndhG-ndhI_p3	ndhG-ndhI	3	AGTAATACTACCCCCAGCCC	AACAATACGAGCTGCACGAT	20	20	55.0	45.0	57.9	58.0	0.0	25.3	0.123952	115909	116149	826-826	0.500000	59.0
ndhG-ndhI_p4	ndhG-ndhI	4	GTAATACTACCCCCAGCCCT	AACAATACGAGCTGCACGAT	20	20	55.0	45.0	57.9	58.0	0.0	25.3	0.123952	115909	116149	825-825	0.500000	59.0
ndhG-ndhI_p5	ndhG-ndhI	5	GTAATACTACCCCCAGCCCT	TGTTCCCTATGGTGACTGGT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.152670	115909	116149	865-865	0.500000	58.8
ndhI_p1	ndhI	1	AGTAATACTACCCCCAGCCC	CGTATGAAGCCGAGGTTTCA	20	20	55.0	50.0	57.9	58.0	0.0	20.7	0.118390	116150	116689	1572-1572	0.500000	59.1
ndhI_p2	ndhI	2	GTAATACTACCCCCAGCCCT	CGTATGAAGCCGAGGTTTCA	20	20	55.0	50.0	57.9	58.0	0.0	20.7	0.118390	116150	116689	1571-1571	0.500000	59.1
ndhI_p3	ndhI	3	AGTAATACTACCCCCAGCCC	TACCCGAAGCAGAAGAGGAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.180786	116150	116689	1356-1356	0.500000	58.6
ndhI_p4	ndhI	4	GTAATACTACCCCCAGCCCT	TACCCGAAGCAGAAGAGGAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.180786	116150	116689	1355-1355	0.500000	58.6
ndhI_p5	ndhI	5	GTAATACTACCCCCAGCCCT	TTACCCGAAGCAGAAGAGGA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.180786	116150	116689	1356-1356	0.500000	58.6
ndhA_p1	ndhA	1	ATCGTGCAGCTCGTATTGTT	GGGCATTATTGGTGGAGAGG	20	20	45.0	55.0	58.0	58.0	16.0	0.0	0.023263	116784	118956	2894-2894	0.500000	59.8
ndhA_p2	ndhA	2	ATCGTGCAGCTCGTATTGTT	GGGGCTTATCAGGACCAATG	20	20	45.0	55.0	58.0	58.0	16.0	0.0	0.023263	116784	118956	2862-2862	0.500000	59.8
ndhA_p3	ndhA	3	ACCAGTCACCATAGGGAACA	GGGCATTATTGGTGGAGAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051981	116784	118956	2854-2854	0.500000	59.6
ndhA_p4	ndhA	4	ACCAGTCACCATAGGGAACA	GGGGCTTATCAGGACCAATG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051981	116784	118956	2822-2822	0.500000	59.6
ndhA_p5	ndhA	5	ATCGTGCAGCTCGTATTGTT	CGGCGAAATGAGAGAATCCA	20	20	45.0	50.0	58.0	58.1	16.0	0.0	0.070171	116784	118956	2717-2717	0.500000	59.4
ndhH_p1	ndhH	1	CCAACGGACCAGCATATTCA	TATCGCAAAGGGGTCTACGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.270464	118958	120139	1632-1632	0.500000	57.8
ndhH_p2	ndhH	2	AAGTAGTTTTGCCCCGTCTG	TATCGCAAAGGGGTCTACGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.341297	118958	120139	1670-1670	0.500000	57.3
ndhH_p3	ndhH	3	CGGACCAGCATATTCAGGAC	TATCGCAAAGGGGTCTACGA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.362426	118958	120139	1628-1628	0.500000	57.1
ndhH_p4	ndhH	4	GGGCAATACTTGAAATGGCG	TATCGCAAAGGGGTCTACGA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.441832	118958	120139	1845-1845	0.500000	56.5
ndhH_p5	ndhH	5	GGACCAGCATATTCAGGACC	TATCGCAAAGGGGTCTACGA	20	20	55.0	50.0	57.7	58.2	0.0	0.0	0.495930	118958	120139	1627-1627	0.500000	56.0
ndhH-rps15_p1	ndhH-rps15	1	CATTGGTCCTGATAAGCCCC	TATCGCAAAGGGGTCTACGA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.246624	120140	120246	907-907	0.500000	58.0
ndhH-rps15_p2	ndhH-rps15	2	CCTCTCCACCAATAATGCCC	TATCGCAAAGGGGTCTACGA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.246624	120140	120246	875-875	0.500000	58.0
ndhH-rps15_p3	ndhH-rps15	3	TTTCCAATCGTTCTGGTGCA	TATCGCAAAGGGGTCTACGA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.259241	120140	120246	509-509	0.500000	57.9
ndhH-rps15_p4	ndhH-rps15	4	GCCATAAAAGGACCCAACCA	TATCGCAAAGGGGTCTACGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.301871	120140	120246	606-606	0.500000	57.6
ndhH-rps15_p5	ndhH-rps15	5	TGCCATAAAAGGACCCAACC	TATCGCAAAGGGGTCTACGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.301871	120140	120246	607-607	0.500000	57.6
rps15_p1	rps15	1	CCTCTCCACCAATAATGCCC	TCCCAGACGTCGTAAAGAGT	20	20	55.0	50.0	58.0	58.1	0.0	10.5	0.111527	120247	120519	1765-1765	0.500000	59.1
rps15_p2	rps15	2	TTTCCAATCGTTCTGGTGCA	TCCCAGACGTCGTAAAGAGT	20	20	45.0	50.0	58.0	58.1	0.0	10.5	0.124144	120247	120519	1399-1399	0.500000	59.0
rps15_p3	rps15	3	GCCATAAAAGGACCCAACCA	TCCCAGACGTCGTAAAGAGT	20	20	50.0	50.0	58.1	58.1	0.0	10.5	0.166774	120247	120519	1496-1496	0.500000	58.7
rps15_p4	rps15	4	TGCCATAAAAGGACCCAACC	TCCCAGACGTCGTAAAGAGT	20	20	50.0	50.0	58.1	58.1	0.0	10.5	0.166774	120247	120519	1497-1497	0.500000	58.7
rps15_p5	rps15	5	GGCGGCTTCGAATAGATCAT	TCCCAGACGTCGTAAAGAGT	20	20	50.0	50.0	57.9	58.1	0.0	10.5	0.184464	120247	120519	1572-1572	0.500000	58.5
rps15-ycf1_p1	rps15-ycf1	1	TTTCCAATCGTTCTGGTGCA	TCCCAGACGTCGTAAAGAGT	20	20	45.0	50.0	58.0	58.1	0.0	10.5	0.124144	120520	120975	1399-1399	0.500000	59.0
rps15-ycf1_p2	rps15-ycf1	2	GCCATAAAAGGACCCAACCA	TCCCAGACGTCGTAAAGAGT	20	20	50.0	50.0	58.1	58.1	0.0	10.5	0.166774	120520	120975	1496-1496	0.500000	58.7
rps15-ycf1_p3	rps15-ycf1	3	TGCCATAAAAGGACCCAACC	TCCCAGACGTCGTAAAGAGT	20	20	50.0	50.0	58.1	58.1	0.0	10.5	0.166774	120520	120975	1497-1497	0.500000	58.7
rps15-ycf1_p4	rps15-ycf1	4	TTTCCAATCGTTCTGGTGCA	TCGAACCAATCCGTCTCTCT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.190241	120520	120975	1601-1601	0.500000	58.5
rps15-ycf1_p5	rps15-ycf1	5	TTTCCAATCGTTCTGGTGCA	AAAGAATAAGCACCACGGGG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.191547	120520	120975	1525-1525	0.500000	58.5
ycf1_p1	ycf1	1	AGTTCTGTCTCCTTTCCCCA	AAAAAGGTCAGAAGCCTGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119479	124006	124125	405-405	0.500000	59.0
ycf1_p2	ycf1	2	AGTTCTGTCTCCTTTCCCCA	CGCACAACAATCCGACTTTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167932	124006	124125	699-699	0.500000	58.7
ycf1_p3	ycf1	3	GCAGTTCTGTCTCCTTTCCC	AAAAAGGTCAGAAGCCTGGG	20	20	55.0	50.0	58.2	58.0	1.8	0.0	0.186283	124006	124125	407-407	0.500000	58.5
ycf1_p4	ycf1	4	GGCAGTTCTGTCTCCTTTCC	AAAAAGGTCAGAAGCCTGGG	20	20	55.0	50.0	58.2	58.0	1.8	0.0	0.186283	124006	124125	408-408	0.500000	58.5
ycf1_p5	ycf1	5	GGGCAGTTCTGTCTCCTTTC	AAAAAGGTCAGAAGCCTGGG	20	20	55.0	50.0	58.2	58.0	4.4	0.0	0.186283	124006	124125	409-409	0.500000	58.5