Hotspot

rps15

SSC · 127438-127710 · 273 bp

Back to Gironniera

Pi 0.44

MarkerSeek score 77.20

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 113
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GCCCCTAGACGTCGTATAGA	AGCCATGAGGTAAATCAGCG	55.0	50.0	57.8	58.0	1586 bp	0.5	58.3
rps15_p2	GCCCCTAGACGTCGTATAGA	CATGAGGTAAATCAGCGGCT	55.0	50.0	57.8	58.0	1583 bp	0.5	58.3
rps15_p3	AGCCCCTAGACGTCGTATAG	AGCCATGAGGTAAATCAGCG	55.0	50.0	57.8	58.0	1587 bp	0.5	58.2
rps15_p4	AGCCCCTAGACGTCGTATAG	CATGAGGTAAATCAGCGGCT	55.0	50.0	57.8	58.0	1584 bp	0.5	58.2
rps15_p5	GCCCCTAGACGTCGTATAGA	GATGAGTCGAAGAACGCCAT	55.0	50.0	57.8	58.1	1162 bp	0.5	58.0