Hotspot

psaC

SSC · 133628-133873 · 246 bp

Back to Gironniera

Pi 0.5

MarkerSeek score 76.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 115
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCTCACATGACTCGATCCGA	GGACCAGAAAAACCCGTTCT	50.0	50.0	58.0	58.0	498 bp	0.5	59.5
psaC_p2	TCTCACATGACTCGATCCGA	CGTCAGCAAAATGGGTCCTA	50.0	50.0	58.0	57.9	797 bp	0.5	58.8
psaC_p3	TCTCACATGACTCGATCCGA	TTGGACCAGAAAAACCCGTT	50.0	45.0	58.0	57.9	500 bp	0.5	58.5
psaC_p4	TCTCACATGACTCGATCCGA	GTGATAAACCCCGTCAGCAA	50.0	50.0	58.0	58.2	808 bp	0.5	58.2
psaC_p5	TCTCACATGACTCGATCCGA	CGTTTCTTTCCTCCCCACAT	50.0	50.0	58.0	57.8	997 bp	0.5	58.1