Hotspot

ndhF

SSC · 139131-141389 · 2259 bp

Back to Gironniera

Pi 0.435

MarkerSeek score 75.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 686
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGCCGGTAACTCAGAAACA	GCAGAATACCGTCACCCATT	50.0	50.0	57.5	58.0	2643 bp	0.5	56.1
ndhF_p2	GAGCCGGTAACTCAGAAACA	AAGAGGGATCCACCGAAGAA	50.0	50.0	57.5	58.0	2532 bp	0.5	56.0
ndhF_p3	GAGCCGGTAACTCAGAAACA	AGGGATCCACCGAAGAAGAT	50.0	50.0	57.5	57.8	2529 bp	0.5	54.9
ndhF_p4	AGAGCCGGTAACTCAGAAAC	GCAGAATACCGTCACCCATT	50.0	50.0	57.3	58.0	2644 bp	0.5	53.7
ndhF_p5	AGAGCCGGTAACTCAGAAAC	AAGAGGGATCCACCGAAGAA	50.0	50.0	57.3	58.0	2533 bp	0.5	53.7