Hotspot

ndhD

SSC · 134002-135504 · 1503 bp

Back to Gironniera

Pi 0.463

MarkerSeek score 79.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 555
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	TCAAAGTAATGGCTTCGCGA	50.0	45.0	58.0	57.9	1928 bp	0.5	59.2
ndhD_p2	GGGACGGGTGTAAAGCTAAG	TCAAAGTAATGGCTTCGCGA	55.0	45.0	58.0	57.9	1957 bp	0.5	59.1
ndhD_p3	AGAACGGGTTTTTCTGGTCC	TCAAAGTAATGGCTTCGCGA	50.0	45.0	58.0	57.9	1714 bp	0.5	59.1
ndhD_p4	AGATGTGAATCCGCTTGTCC	TCAAAGTAATGGCTTCGCGA	50.0	45.0	58.0	57.9	1892 bp	0.5	59.1
ndhD_p5	TCTCACATGACTCGATCCGA	TCAAAGTAATGGCTTCGCGA	50.0	45.0	58.0	57.9	2192 bp	0.5	59.0