Hotspot

rpl32-ndhF

SSC · 137897-139130 · 1234 bp

Back to Gironniera

Pi 0.424

MarkerSeek score 80.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1234
variable_sites: 443
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GGTAGGTATTGGTCTGGGCT	ATGAAGGGGAAATTGGGCAG	55.0	50.0	58.5	58.1	2330 bp	0.5	55.0
rpl32-ndhF_p2	GGTAGGTATTGGTCTGGGCT	GAAGGGGAAATTGGGCAGAT	55.0	50.0	58.5	57.8	2328 bp	0.5	54.7
rpl32-ndhF_p3	GGTAGGTATTGGTCTGGGCT	CGGTCGTGTGAACCAAAAAC	55.0	50.0	58.5	58.2	2096 bp	0.5	54.6
rpl32-ndhF_p4	GGTAGGTATTGGTCTGGGCT	GGTCGTGTGAACCAAAAACC	55.0	50.0	58.5	57.8	2095 bp	0.5	54.5
rpl32-ndhF_p5	AGGTATTGGTCTGGGCTAGT	ATGAAGGGGAAATTGGGCAG	50.0	50.0	57.4	58.1	2327 bp	0.5	54.4