Hotspot

ndhI-ndhG

SSC · 131894-132276 · 383 bp

Back to Gironniera

Pi 0.426

MarkerSeek score 78.60

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 383
variable_sites: 152
pi: 0.426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	ACCCGTCGTTGATTGGAAAT	ACCAGCAAAGTAACCCCATC	45.0	50.0	57.8	57.8	962 bp	0.5	56.7
ndhI-ndhG_p2	ACCCGTCGTTGATTGGAAAT	ATCCCCAAGGGTCCAAAGAT	45.0	50.0	57.8	58.3	945 bp	0.5	56.0
ndhI-ndhG_p3	AAATTGCCCTGGGTCGTTTA	ACCAGCAAAGTAACCCCATC	45.0	50.0	57.7	57.8	780 bp	0.5	55.9
ndhI-ndhG_p4	ACCCGTCGTTGATTGGAAAT	AGTTGTGCAGCAGCTACAAA	45.0	45.0	57.8	58.3	842 bp	0.5	55.9
ndhI-ndhG_p5	ACCCGTCGTTGATTGGAAAT	TCCCGTACCATGACGTATCT	45.0	50.0	57.8	57.6	1021 bp	0.5	55.6