Hotspot

rpoC2-rpoB

LSC · 19334-35870 · 16537 bp

Back to Gironniera

Pi 0.0422

MarkerSeek score 52.30

Primer available yes

Label rpoC2-rpoB

Feature Metadata

label_name: rpoC2-rpoB
feature_type: igs
region: LSC
length_bp: 16537
variable_sites: 278
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoB_p1	CCCTCCCTGTCATCGTATCT	TGCCCCCTTATCTACTACGG	55.0	55.0	58.0	58.0	1822 bp	0.5	59.9
rpoC2-rpoB_p2	TCCCTCCCTGTCATCGTATC	TGCCCCCTTATCTACTACGG	55.0	55.0	58.0	58.0	1823 bp	0.5	59.9
rpoC2-rpoB_p3	GTCTTGTCAACAACGCTTCG	TGCCCCCTTATCTACTACGG	50.0	55.0	58.0	58.0	1227 bp	0.5	59.9
rpoC2-rpoB_p4	CCCTCCCTGTCATCGTATCT	GAACTACTGAAGCTGCTCCC	55.0	55.0	58.0	58.0	1664 bp	0.5	59.8
rpoC2-rpoB_p5	CCCTCCCTGTCATCGTATCT	GGAACTACTGAAGCTGCTCC	55.0	55.0	58.0	58.0	1665 bp	0.5	59.8