Hotspot

ycf1

SSC · 122258-127885 · 5628 bp

Back to Girardinia

Pi 0.0232

MarkerSeek score 58.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 353
pi: 0.0232
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCAAGGTTTCAGACGAAA	AAAAGTTCCTCGGTGGTCAC	45.0	50.0	58.0	58.0	852-876 bp	1.0	86.9
ycf1_p2	GCCCATTCAGGAGGTTGAAA	TTCGGCGAGACATAATTGCA	50.0	45.0	58.1	58.0	454-460 bp	1.0	85.3
ycf1_p3	TGCCAAGGTTTCAGACGAAA	TTCGGCGAGACATAATTGCA	45.0	45.0	58.0	58.0	601-607 bp	1.0	85.2
ycf1_p4	AATCCGTTAAATCCTCGGCC	TTCGGCGAGACATAATTGCA	50.0	45.0	58.0	58.0	471-477 bp	0.666667	72.0
ycf1_p5	AATCCGTTAAATCCTCGGCC	AAAAGTTCCTCGGTGGTCAC	50.0	50.0	58.0	58.0	722-746 bp	0.666667	71.9