Hotspot

rps4-trnT-UGU

LSC · 45699-46129 · 431 bp

Back to Girardinia

Pi 0.0251

MarkerSeek score 60.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 30
pi: 0.0251
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CGGGGCGTAATTGGTTTCTA	ATTATGTGAGCCCGCTTAGC	50.0	50.0	58.0	58.1	628-675 bp	1.0	82.5
rps4-trnT-UGU_p2	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	609-656 bp	1.0	82.4
rps4-trnT-UGU_p3	CGGGGCGTAATTGGTTTCTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.0	58.2	578-625 bp	1.0	82.4
rps4-trnT-UGU_p4	CCCCCAGACGACGTATTTTT	ATTATGTGAGCCCGCTTAGC	50.0	50.0	57.9	58.1	562-609 bp	1.0	82.2
rps4-trnT-UGU_p5	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	543-590 bp	1.0	82.2