Hotspot

trnE-UUC-trnT-GGU

LSC · 30336-30894 · 559 bp

Back to Girardinia

Pi 0.0122
MarkerSeek score 58.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
559
variable_sites
17
pi
0.0122
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 644-653 bp 1.0 81.3
trnE-UUC-trnT-GGU_p2 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 646-655 bp 1.0 81.2
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 700-709 bp 1.0 80.9
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 625-634 bp 1.0 80.9
trnE-UUC-trnT-GGU_p5 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 623-632 bp 1.0 80.9