Hotspot

rps16-trnQ-UUG

LSC · 5978-6670 · 693 bp

Back to Girardinia

Pi 0.0193

MarkerSeek score 68.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 37
pi: 0.0193
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	766-821 bp	1.0	84.4
rps16-trnQ-UUG_p2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	780-835 bp	1.0	82.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	787-842 bp	1.0	80.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	770-825 bp	1.0	79.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	796-851 bp	1.0	79.3