Hotspot

trnH-GUG-psbA

LSC · 72-251 · 180 bp

Back to Girardinia

Pi 0.0314

MarkerSeek score 70.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 14
pi: 0.0314
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAACCGTGCTAATCTTGGT	55.0	45.0	58.1	57.1	293-303 bp	1.0	78.7
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	TCAACCGTGCTAATCTTGGT	50.0	45.0	57.9	57.1	306-316 bp	1.0	78.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCAACCGTGCTAATCTTGGT	50.0	45.0	57.9	57.1	307-317 bp	1.0	78.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TCAACCGTGCTAATCTTGGT	50.0	45.0	57.9	57.1	308-318 bp	1.0	78.3
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TCCCTCTAGACTTAGCTGCT	55.0	50.0	58.1	56.9	239-249 bp	1.0	77.9