primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAACCGTGCTAATCTTGGT	20	20	55.0	45.0	58.1	57.1	0.0	0.0	0.980183	72	251	293-303	1.000000	78.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	TCAACCGTGCTAATCTTGGT	20	20	50.0	45.0	57.9	57.1	0.0	0.0	0.986052	72	251	306-316	1.000000	78.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TCAACCGTGCTAATCTTGGT	20	20	50.0	45.0	57.9	57.1	0.0	0.0	0.984544	72	251	307-317	1.000000	78.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TCAACCGTGCTAATCTTGGT	20	20	50.0	45.0	57.9	57.1	0.0	0.0	0.986052	72	251	308-318	1.000000	78.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TCCCTCTAGACTTAGCTGCT	20	20	55.0	50.0	58.1	56.9	0.0	7.6	1.263417	72	251	239-249	1.000000	77.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5978	6670	766-821	1.000000	84.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	5978	6670	780-835	1.000000	82.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.672468	5978	6670	787-842	1.000000	80.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5978	6670	770-825	1.000000	79.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5978	6670	796-851	1.000000	79.3
psbK-psbI_p1	psbK-psbI	1	CAAGCCGCTGTAAGTTTTCG	TCCGAAGATGAATAAAGAGACA	20	22	50.0	36.4	58.0	54.7	0.0	0.0	5.380982	7295	7770	550-566	1.000000	46.9
psbK-psbI_p2	psbK-psbI	2	CAAGCCGCTGTAAGTTTTCG	AGGAATCCGAAGATGAATAAAGAGA	20	25	50.0	36.0	58.0	57.6	0.0	0.0	5.416375	7295	7770	555-571	1.000000	46.9
psbK-psbI_p3	psbK-psbI	3	CAAGCCGCTGTAAGTTTTCG	GGAATCCGAAGATGAATAAAGAGAC	20	25	50.0	40.0	58.0	57.6	0.0	0.0	5.464342	7295	7770	554-570	1.000000	46.9
psbK-psbI_p4	psbK-psbI	4	CAAGCCGCTGTAAGTTTTCG	AGATAGGAATCCGAAGATGA	20	20	50.0	40.0	58.0	52.6	0.0	0.0	5.442925	7295	7770	559-575	1.000000	46.8
psbK-psbI_p5	psbK-psbI	5	CAAGCCGCTGTAAGTTTTCG	GGATCATTAGATAGGAATCCGAAGA	20	25	50.0	40.0	58.0	57.6	0.0	0.0	5.461014	7295	7770	567-583	1.000000	46.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8098	8723	687-710	1.000000	57.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8098	8723	686-709	1.000000	57.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8098	8723	690-713	1.000000	56.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8098	8723	689-712	1.000000	56.3
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8098	8723	688-711	1.000000	56.3
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CCAGCGGTATTCAAGCTCTT	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.068225	13519	14131	657-722	1.000000	88.4
atpH-atpI_p2	atpH-atpI	2	AAAAAGCAAAAGCAGCAGCA	CCAGCGGTATTCAAGCTCTT	20	20	40.0	50.0	58.0	58.0	3.2	0.0	0.056842	13519	14131	941-1006	1.000000	87.4
atpH-atpI_p3	atpH-atpI	3	AAAAGCAAAAGCAGCAGCAA	CCAGCGGTATTCAAGCTCTT	20	20	40.0	50.0	58.0	58.0	3.2	0.0	0.056842	13519	14131	940-1005	1.000000	87.4
atpH-atpI_p4	atpH-atpI	4	AAAGCAAAAGCAGCAGCAAA	CCAGCGGTATTCAAGCTCTT	20	20	40.0	50.0	58.0	58.0	3.2	0.0	0.056842	13519	14131	939-1004	1.000000	87.4
atpH-atpI_p5	atpH-atpI	5	GCGGATGGCCAATAACCTAA	CCAGCGGTATTCAAGCTCTT	20	20	50.0	50.0	58.0	58.0	11.0	0.0	0.054179	13519	14131	1375-1437	1.000000	86.9
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGTTCATTCTAGTCCCTACTGC	20	22	50.0	45.5	58.2	57.3	0.0	0.0	2.889204	28537	29078	693-698	1.000000	61.9
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGTTCATTCTAGTCCCTACTGC	20	22	50.0	45.5	57.6	57.3	0.0	0.0	3.088718	28537	29078	632-637	1.000000	60.8
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	GTTCATTCTAGTCCCTACTGCT	20	22	50.0	45.5	58.2	57.0	0.0	0.0	3.163260	28537	29078	692-697	1.000000	59.8
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	GTTCATTCTAGTCCCTACTGCT	20	22	50.0	45.5	57.6	57.0	0.0	0.0	3.362775	28537	29078	631-636	1.000000	58.6
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	GTTCATTCTAGTCCCTACTGCTT	20	23	50.0	43.5	58.2	57.7	0.0	0.0	3.497529	28537	29078	692-697	1.000000	57.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TAGTAAGTCTCGCTTGGGCT	CAAAAATTCGCCGATCCACC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.243283	29184	29663	1669-1688	0.833333	76.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TCGGACGAGTCAAAACTGTC	CAAAAATTCGCCGATCCACC	20	20	50.0	50.0	57.9	58.1	5.6	0.0	0.221758	29184	29663	1412-1412	0.166667	44.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TCGGACGAGTCAAAACTGTC	CCAAAAATTCGCCGATCCAC	20	20	50.0	50.0	57.9	58.1	5.6	0.0	0.221758	29184	29663	1413-1413	0.166667	44.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	CGGACGAGTCAAAACTGTCT	CAAAAATTCGCCGATCCACC	20	20	50.0	50.0	57.9	58.1	5.6	0.0	0.227599	29184	29663	1411-1411	0.166667	44.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	CGGACGAGTCAAAACTGTCT	CCAAAAATTCGCCGATCCAC	20	20	50.0	50.0	57.9	58.1	5.6	0.0	0.227599	29184	29663	1412-1412	0.166667	44.8
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30336	30894	644-653	1.000000	81.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30336	30894	646-655	1.000000	81.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30336	30894	700-709	1.000000	80.9
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30336	30894	625-634	1.000000	80.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30336	30894	623-632	1.000000	80.9
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CGGGGCGTAATTGGTTTCTA	ATTATGTGAGCCCGCTTAGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.142768	45699	46129	628-675	1.000000	82.5
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.159743	45699	46129	609-656	1.000000	82.4
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CGGGGCGTAATTGGTTTCTA	GATGGTCATCGGTTCGACTC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.185554	45699	46129	578-625	1.000000	82.4
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CCCCCAGACGACGTATTTTT	ATTATGTGAGCCCGCTTAGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.219302	45699	46129	562-609	1.000000	82.2
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.236277	45699	46129	543-590	1.000000	82.2
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	47290	47782	540-736	1.000000	83.1
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	47290	47782	576-772	1.000000	82.3
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	47290	47782	591-787	1.000000	81.9
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	20	20	50.0	50.0	57.5	58.4	0.0	0.0	0.854756	47290	47782	592-788	1.000000	79.5
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	47290	47782	573-769	1.000000	79.1
ndhF-rpl32_p1	ndhF-rpl32	1	ACTGGAAGGGGAATAAAAGGT	TCCAAAGATTTTTACGAATACG	21	22	42.9	31.8	56.5	52.9	0.0	0.0	9.558618	111044	111860	892-931	1.000000	48.4
ndhF-rpl32_p2	ndhF-rpl32	2	AGGAACTGGAAGGGGAATAA	TCCAAAGATTTTTACGAATACG	20	22	45.0	31.8	55.4	52.9	0.0	0.0	9.673228	111044	111860	896-935	1.000000	48.3
ndhF-rpl32_p3	ndhF-rpl32	3	ACTGGAAGGGGAATAAAAGGT	TTCCAAAGATTTTTACGAATACG	21	23	42.9	30.4	56.5	53.8	0.0	0.0	9.736676	111044	111860	893-932	1.000000	48.3
ndhF-rpl32_p4	ndhF-rpl32	4	ACTGGAAGGGGAATAAAAGGT	CCTTTCTTTTTCCAAAGATTTTTACGA	21	27	42.9	29.6	56.5	57.7	0.0	0.0	9.805582	111044	111860	902-941	1.000000	48.3
ndhF-rpl32_p5	ndhF-rpl32	5	AACTGGAAGGGGAATAAAAGGT	TCCAAAGATTTTTACGAATACG	22	22	40.9	31.8	57.2	52.9	0.0	0.0	9.821147	111044	111860	893-932	1.000000	48.3
rps15-ycf1_p1	rps15-ycf1	1	CGGAGGCCTCTCTGAGATAA	GCAGTCGTTTCAGTCTGCTA	20	20	55.0	50.0	58.0	57.9	31.4	8.5	0.077699	121868	122257	564-604	1.000000	89.2
rps15-ycf1_p2	rps15-ycf1	2	CGGAGGCCTCTCTGAGATAA	TGTCTCCTGCTTAGACTGCT	20	20	55.0	50.0	58.0	58.1	31.4	12.7	0.081456	121868	122257	1162-1198	1.000000	86.9
rps15-ycf1_p3	rps15-ycf1	3	CGGAGGCCTCTCTGAGATAA	TTATCCCCTAGGCGTCGTAG	20	20	55.0	55.0	58.0	58.1	31.4	2.1	0.110116	121868	122257	805-841	0.833333	81.3
rps15-ycf1_p4	rps15-ycf1	4	CGGAGGCCTCTCTGAGATAA	AGGTCCCTAGAAAAAGGCCT	20	20	55.0	50.0	58.0	57.9	31.4	0.0	0.072970	121868	122257	959-959	0.166667	46.1
rps15-ycf1_p5	rps15-ycf1	5	ATGAGTCGAAGAACACCGTG	AGGTCCCTAGAAAAAGGCCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.127256	121868	122257	1266-1266	0.166667	45.6
ycf1_p1	ycf1	1	TGCCAAGGTTTCAGACGAAA	AAAAGTTCCTCGGTGGTCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.082389	125479	125598	852-876	1.000000	86.9
ycf1_p2	ycf1	2	GCCCATTCAGGAGGTTGAAA	TTCGGCGAGACATAATTGCA	20	20	50.0	45.0	58.1	58.0	0.0	10.5	0.093782	125479	125598	454-460	1.000000	85.3
ycf1_p3	ycf1	3	TGCCAAGGTTTCAGACGAAA	TTCGGCGAGACATAATTGCA	20	20	45.0	45.0	58.0	58.0	0.0	10.5	0.062699	125479	125598	601-607	1.000000	85.2
ycf1_p4	ycf1	4	AATCCGTTAAATCCTCGGCC	TTCGGCGAGACATAATTGCA	20	20	50.0	45.0	58.0	58.0	0.0	10.5	0.053966	125479	125598	471-477	0.666667	72.0
ycf1_p5	ycf1	5	AATCCGTTAAATCCTCGGCC	AAAAGTTCCTCGGTGGTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073656	125479	125598	722-746	0.666667	71.9