Hotspot

ycf1

SSC · 128702-134317 · 5616 bp

Back to Gillenia

Pi 0.0155

MarkerSeek score 50.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5616
variable_sites: 87
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACATTCATAACAAGGGCTTCCT	ACAAAGAAAGATGTCTCTGT	40.9	35.0	57.7	52.0	168 bp	1.0	43.8
ycf1_p2	ACATTCATAACAAGGGCTTCCT	ACAAAGAAAGATGTCTCTGTT	40.9	33.3	57.7	53.0	168 bp	1.0	43.8
ycf1_p3	ACATTCATAACAAGGGCTTCC	ACAAAGAAAGATGTCTCTGT	42.9	35.0	56.2	52.0	168 bp	1.0	43.8
ycf1_p4	ACATTCATAACAAGGGCTTCCT	TGATACAAAGAAAGATGTCTCTGT	40.9	33.3	57.7	55.8	172 bp	1.0	43.7
ycf1_p5	CGAGTACATTCATAACAAGGGC	ACAAAGAAAGATGTCTCTGT	45.5	35.0	57.2	52.0	173 bp	1.0	43.7