Hotspot

ndhE-ndhG

SSC · 122671-122921 · 251 bp

Back to Gillenia

Pi 0.0206

MarkerSeek score 67.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 5
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ATTCCGGCTCGTGATCAATC	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.1	58.2	355-362 bp	1.0	80.5
ndhE-ndhG_p2	GGCTCTAACCATATTCCGGC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.2	58.2	367-374 bp	1.0	79.9
ndhE-ndhG_p3	CCATATTCCGGCTCGTGATC	TTGATAGGTGCAATTGCCGT	55.0	45.0	58.3	58.2	359-366 bp	1.0	79.3
ndhE-ndhG_p4	CGGCTCGTGATCAATCCATA	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.6	58.2	351-358 bp	1.0	78.1
ndhE-ndhG_p5	ATTCCGGCTCGTGATCAATC	AGTCGCTTTGATAGGTGCAA	50.0	45.0	58.1	57.5	362-369 bp	1.0	78.0