Hotspot

rpl32-trnL-UAG

SSC · 117659-118824 · 1166 bp

Back to Gillenia

Pi 0.194
MarkerSeek score 88.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1166
variable_sites
216
pi
0.194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1288-1400 bp 1.0 88.8
rpl32-trnL-UAG_p2 TAGGGCAGCATTGAAAGCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1290-1402 bp 1.0 88.4
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.8 57.8 1281-1393 bp 1.0 88.3
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 1287-1399 bp 1.0 88.2
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 1286-1398 bp 1.0 88.2