Hotspot

ndhF-rpl32

SSC · 116407-117499 · 1093 bp

Back to Gillenia

Pi 0.0172

MarkerSeek score 70.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1093
variable_sites: 18
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	1261-1289 bp	1.0	74.4
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	40.0	45.0	57.0	57.8	1259-1287 bp	1.0	74.0
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	40.0	55.0	57.0	58.3	1248-1276 bp	1.0	73.1
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	40.0	45.0	57.0	57.6	1258-1286 bp	1.0	72.2
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	1262-1290 bp	1.0	72.1