Hotspot

rps8-rpl14

LSC · 84111-84272 · 162 bp

Back to Gillenia

Pi 0.037

MarkerSeek score 67.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 6
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGTCAGCAATAGTGTCTTTACTCA	AATCGCCCGGGAATTAAGAC	37.5	50.0	57.9	58.0	254 bp	1.0	48.8
rps8-rpl14_p2	TGTCAGCAATAGTGTCTTTACTCA	CAATCGCCCGGGAATTAAGA	37.5	50.0	57.9	58.0	255 bp	1.0	48.8
rps8-rpl14_p3	TGTCAGCAATAGTGTCTTTACTCA	GCCCGGGAATTAAGACAGTT	37.5	50.0	57.9	57.9	250 bp	1.0	48.2
rps8-rpl14_p4	AGATGGTATTCGAACTGTTCT	AATCGCCCGGGAATTAAGAC	38.1	50.0	54.4	58.0	313 bp	1.0	44.3
rps8-rpl14_p5	AGATGGTATTCGAACTGTTCT	CAATCGCCCGGGAATTAAGA	38.1	50.0	54.4	58.0	314 bp	1.0	44.3