Hotspot

trnF-GAA-ndhJ

LSC · 51726-52459 · 734 bp

Back to Gillenia

Pi 0.0236

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 17
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1275-1285 bp	1.0	81.8
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AAAACCCGAGGATTGGCATT	55.0	45.0	58.0	58.1	1179-1189 bp	1.0	81.6
trnF-GAA-ndhJ_p3	CTATCCCCAAAATGACCCGG	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1685-1696 bp	1.0	81.5
trnF-GAA-ndhJ_p4	CACAAATGGATCTGAGCGGA	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1571-1582 bp	1.0	81.3
trnF-GAA-ndhJ_p5	TCACAAATGGATCTGAGCGG	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.0	58.0	1572-1583 bp	1.0	81.3