Hotspot

rps4-trnT-UGU

LSC · 49132-49531 · 400 bp

Back to Gillenia

Pi 0.0347

MarkerSeek score 66.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 13
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	466-490 bp	1.0	82.9
rps4-trnT-UGU_p2	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	471-495 bp	1.0	82.2
rps4-trnT-UGU_p3	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	434-458 bp	1.0	82.2
rps4-trnT-UGU_p4	GAAACGAGGCCCTCTGTAAC	ATTTAGCCCGCTTAGCTCAG	55.0	50.0	58.3	57.8	481-505 bp	1.0	81.9
rps4-trnT-UGU_p5	TTTTGAAACGAGGCCCTCTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	470-494 bp	1.0	81.9