Hotspot

psaA-ycf3

LSC · 44564-45267 · 704 bp

Back to Gillenia

Pi 0.017

MarkerSeek score 68.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 12
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	856-869 bp	1.0	77.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	896-909 bp	1.0	75.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCTATAGCGCT	50.0	45.0	57.9	57.3	865-878 bp	1.0	74.6
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.5	57.6	876-889 bp	1.0	74.5
psaA-ycf3_p5	TTTAACTTCTGGTTCCGGCG	GCTATAGCGCTTACTCCTGG	50.0	55.0	58.5	57.6	809-822 bp	1.0	74.5