Hotspot

trnD-GUC-trnY-GUA

LSC · 32668-33108 · 441 bp

Back to Gillenia

Pi 0.0147

MarkerSeek score 67.90

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 6
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGGATTCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	564-567 bp	1.0	82.7
trnD-GUC-trnY-GUA_p2	ATTTGGATTCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	563-566 bp	1.0	82.7
trnD-GUC-trnY-GUA_p3	ATTTGGATTCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	585-588 bp	1.0	81.8
trnD-GUC-trnY-GUA_p4	ATTTGGATTCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	584-587 bp	1.0	81.6
trnD-GUC-trnY-GUA_p5	ATTTGGATTCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	618-621 bp	1.0	81.3