Hotspot

atpF-atpH

LSC · 13533-13993 · 461 bp

Back to Gillenia

Pi 0.0197

MarkerSeek score 68.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 9
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CTGGTTGTCGCATTAGCTCT	50.0	50.0	57.9	58.0	575-609 bp	1.0	80.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTCGCATTAG	50.0	50.0	57.9	57.9	579-613 bp	1.0	80.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	ACTGGTTGTCGCATTAGCTC	50.0	50.0	57.9	58.3	576-610 bp	1.0	78.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GACTGGTTGTCGCATTAGCT	50.0	50.0	57.9	58.3	577-611 bp	1.0	78.6
atpF-atpH_p5	GATGGCCAATAACCCAAGGA	CTGGTTGTCGCATTAGCTCT	50.0	50.0	57.5	58.0	553-587 bp	1.0	78.1