Hotspot

psbK-psbl

LSC · 7827-8083 · 257 bp

Back to Gillenia

Pi 0.0558

MarkerSeek score 69.90

Primer available yes

Label psbK-psbl

Feature Metadata

label_name: psbK-psbl
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 14
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbl_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	613-619 bp	1.0	83.0
psbK-psbl_p2	CGTGGGTCAGGTATGTTCTG	TGGACTAAAGCGTCGGATTG	55.0	50.0	58.0	58.0	1253-1260 bp	1.0	81.7
psbK-psbl_p3	CGTGGGTCAGGTATGTTCTG	GTGGACTAAAGCGTCGGATT	55.0	50.0	58.0	58.0	1254-1261 bp	1.0	81.7
psbK-psbl_p4	CGTGGGTCAGGTATGTTCTG	TTTGGAGGAGAGATGGCTGA	55.0	50.0	58.0	58.0	1274-1281 bp	1.0	81.6
psbK-psbl_p5	CGTGGGTCAGGTATGTTCTG	GCCGTGGAACTAAAAAGCAC	55.0	50.0	58.0	58.0	1374 bp	0.5	59.5