Hotspot

trnH-GUG-psbA

LSC · 246-498 · 253 bp

Back to Gillenia

Pi 0.0279
MarkerSeek score 56.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
253
variable_sites
7
pi
0.0279
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 309-313 bp 1.0 79.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 323-327 bp 1.0 78.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 322-326 bp 1.0 78.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 324-328 bp 1.0 78.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTGTTGAAGTTCCATC 55.0 50.0 58.1 57.4 308-312 bp 1.0 76.3