Hotspot

rps3

LSC · 81719-82438 · 720 bp

Back to Gigantochloa

Pi 0.000926
MarkerSeek score 47.00
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
720
variable_sites
2
pi
0.000926
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 ACAGAATGGGAAGACAAGGC TCCATCTAAGATTTCGATTCCCA 50.0 39.1 57.8 57.1 791-793 bp 1.0 47.6
rps3_p2 ACAGAATGGGAAGACAAGGC TCCATCTAAGATTTCGATTCCCAG 50.0 41.7 57.8 58.1 791-793 bp 1.0 45.5
rps3_p3 CAGAATGGGAAGACAAGGCT TCCATCTAAGATTTCGATTCCCA 50.0 39.1 57.5 57.1 790-792 bp 1.0 45.2
rps3_p4 ACAGAATGGGAAGACAAGGC CCATCTAAGATTTCGATTCCCAG 50.0 43.5 57.8 56.8 790-792 bp 1.0 45.1
rps3_p5 ACAGAATGGGAAGACAAGGC CCATCTAAGATTTCGATTCCCA 50.0 40.9 57.8 55.7 790-792 bp 1.0 44.0