Hotspot

rpoC1

LSC · 24594-26642 · 2049 bp

Back to Gigantochloa

Pi 0.000651

MarkerSeek score 45.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2049
variable_sites: 4
pi: 0.000651
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGGGAGGAAGTTTGATCGGA	GCGCGGCAAAAATGATGATA	50.0	45.0	58.0	57.9	2176 bp	1.0	78.8
rpoC1_p2	AGGGAGGAAGTTTGATCGGA	ACTAGCGAAATCGGAATGCC	50.0	50.0	58.0	58.4	2157 bp	1.0	76.4
rpoC1_p3	AGGGAGGAAGTTTGATCGGA	GGCATCGCCGAATCTAACTT	50.0	50.0	58.0	58.4	2127 bp	1.0	76.4
rpoC1_p4	AGGGAGGAAGTTTGATCGGA	ATCTCAATCTGGATTCGCGC	50.0	50.0	58.0	58.5	2192 bp	1.0	75.8
rpoC1_p5	GGGAGGAAGTTTGATCGGAA	GCGCGGCAAAAATGATGATA	50.0	45.0	57.2	57.9	2175 bp	1.0	72.9