Hotspot

cemA

LSC · 60860-61552 · 693 bp

Back to Gigantochloa

Pi 0.00212

MarkerSeek score 44.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 693
variable_sites: 3
pi: 0.00212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	ATAGAGTCAGCGAATGGAGC	TGCTAAAAAGGAAAAGGGCT	50.0	40.0	57.2	55.4	822 bp	1.0	53.1
cemA_p2	ATAGAGTCAGCGAATGGAGC	TGCTAAAAAGGAAAAGGGCTT	50.0	38.1	57.2	56.2	822 bp	1.0	51.6
cemA_p3	ATAGAGTCAGCGAATGGAGC	TTGCTAAAAAGGAAAAGGGCT	50.0	38.1	57.2	56.2	823 bp	1.0	51.6
cemA_p4	GCGAATGGAGCGGATTCATT	TGCTAAAAAGGAAAAGGGCT	50.0	40.0	59.1	55.4	813 bp	1.0	51.2
cemA_p5	AATAGAGTCAGCGAATGGAGC	TGCTAAAAAGGAAAAGGGCT	47.6	40.0	57.9	55.4	823 bp	1.0	50.8