Hotspot

matK-trnK-UUU

LSC · 3238-3929 · 692 bp

Back to Gigantochloa

Pi 0.00212

MarkerSeek score 44.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 3
pi: 0.00212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	47.6	50.0	58.3	57.8	861 bp	1.0	68.5
matK-trnK-UUU_p2	ACTCCTGAAAGAGAAGTGGG	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	861 bp	1.0	66.6
matK-trnK-UUU_p3	CTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	860 bp	1.0	66.6
matK-trnK-UUU_p4	TGAAAGAGAAGTGGGTAGACG	CGTGCGGTGTAATTCCATTG	47.6	50.0	57.1	57.8	856 bp	1.0	63.4
matK-trnK-UUU_p5	AGAGAAGTGGGTAGACGAAG	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.0	57.8	852 bp	1.0	63.0