Hotspot

trnP-UGG-psaJ

LSC · 67388-67809 · 422 bp

Back to Geum

Pi 0.0221
MarkerSeek score 63.40
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
422
variable_sites
25
pi
0.0221
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 572-595 bp 1.0 85.1
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 571-594 bp 1.0 85.1
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 583-606 bp 1.0 84.9
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 582-605 bp 1.0 84.9
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 585-608 bp 1.0 84.9