Hotspot

petA-psbJ

LSC · 63472-64571 · 1100 bp

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Pi 0.0272
MarkerSeek score 61.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1100
variable_sites
78
pi
0.0272
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCTCTTCTTGGCATCCGTTA TGGGTCATATTCTGGATTAGGT 50.0 40.9 58.0 56.0 1037-1339 bp 1.0 57.3
petA-psbJ_p2 GGCATCCGTTATTTTGGCAC TGGGTCATATTCTGGATTAGGT 50.0 40.9 58.1 56.0 1028-1330 bp 1.0 56.9
petA-psbJ_p3 GCTCTTCTTGGCATCCGTTA GGGTCATATTCTGGATTAGGTTCA 50.0 41.7 58.0 57.8 1036-1338 bp 1.0 55.6
petA-psbJ_p4 GCTCTTCTTGGCATCCGTTA TGGGTCATATTCTGGATTAGGTTC 50.0 41.7 58.0 57.8 1037-1339 bp 1.0 55.6
petA-psbJ_p5 GGCATCCGTTATTTTGGCAC GGGTCATATTCTGGATTAGGTTCA 50.0 41.7 58.1 57.8 1027-1329 bp 1.0 55.2