Hotspot

accD-psaI

LSC · 59532-59976 · 445 bp

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Pi 0.0304
MarkerSeek score 67.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
445
variable_sites
31
pi
0.0304
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TACATCCCCTACCACAGGTG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.1 58.0 774-790 bp 1.0 84.3
accD-psaI_p2 AATGGCATTCCCGTAGCAAT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.9 58.0 1025-1041 bp 1.0 83.8
accD-psaI_p3 ATGGCATTCCCGTAGCAATT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.9 58.0 1024-1040 bp 1.0 83.8
accD-psaI_p4 AATGGCATTCCCGTAGCAAT TTGCCGGAAATACTAAGCCC 45.0 50.0 57.9 58.0 1016-1032 bp 1.0 83.7
accD-psaI_p5 ATGGCATTCCCGTAGCAATT TTGCCGGAAATACTAAGCCC 45.0 50.0 57.9 58.0 1015-1031 bp 1.0 83.7