Hotspot

trnD-GUC-trnY-GUA

LSC · 30920-31367 · 448 bp

Back to Geum

Pi 0.0299
MarkerSeek score 64.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
448
variable_sites
32
pi
0.0299
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 518-550 bp 1.0 80.2
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 517-549 bp 1.0 80.2
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 539-571 bp 1.0 79.1
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 538-570 bp 1.0 78.9
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 534-566 bp 1.0 77.8