Hotspot

psbM-trnD-GUC

LSC · 29813-30845 · 1033 bp

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Pi 0.0233
MarkerSeek score 63.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1033
variable_sites
60
pi
0.0233
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 35.0 50.0 52.0 58.5 962-1139 bp 1.0 48.4
psbM-trnD-GUC_p2 ATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 963-1140 bp 1.0 48.4
psbM-trnD-GUC_p3 TGCGAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 962-1139 bp 1.0 48.4
psbM-trnD-GUC_p4 AATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 31.8 50.0 53.6 58.5 964-1141 bp 1.0 48.4
psbM-trnD-GUC_p5 TGCGAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 35.0 50.0 52.0 57.0 963-1140 bp 1.0 48.4