Hotspot

petN-psbM

LSC · 28494-29707 · 1214 bp

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Pi 0.0271
MarkerSeek score 67.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1214
variable_sites
82
pi
0.0271
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG GTAAAAACAGTCAGCCAAGGC 50.0 47.6 58.0 58.0 1322-1365 bp 1.0 79.4
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG CGTAAAAACAGTCAGCCAAGG 50.0 47.6 58.0 57.7 1323-1366 bp 1.0 77.4
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT GTAAAAACAGTCAGCCAAGGC 50.0 47.6 57.6 58.0 1262-1305 bp 1.0 77.2
petN-psbM_p4 AGTAAATCTCGCTTGGGCTG TAAAAACAGTCAGCCAAGGC 50.0 45.0 58.0 56.5 1321-1364 bp 1.0 75.8
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT CGTAAAAACAGTCAGCCAAGG 50.0 47.6 57.6 57.7 1263-1306 bp 1.0 75.2