Hotspot

trnS-GCU-trnG-UCC

LSC · 8594-9236 · 643 bp

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Pi 0.0308
MarkerSeek score 75.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
643
variable_sites
47
pi
0.0308
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 688-754 bp 1.0 66.3
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 687-753 bp 1.0 66.3
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 687-753 bp 1.0 66.3
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 686-752 bp 1.0 66.3
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.2 55.1 741-807 bp 1.0 64.8