Hotspot

psbI-trnS-GCU

LSC · 8339-8505 · 167 bp

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Pi 0.0516
MarkerSeek score 64.40
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
167
variable_sites
21
pi
0.0516
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCTTGTACAACCGCGACAAA TGGACTAAAGCGTCGGATTG 45.0 50.0 58.1 58.0 480-489 bp 1.0 85.5
psbI-trnS-GCU_p2 TTCTTGTACAACCGCGACAA TGGACTAAAGCGTCGGATTG 45.0 50.0 58.1 58.0 481-490 bp 1.0 85.5
psbI-trnS-GCU_p3 TCTTGTACAACCGCGACAAA GTGGACTAAAGCGTCGGATT 45.0 50.0 58.1 58.0 481-490 bp 1.0 85.5
psbI-trnS-GCU_p4 TTCTTGTACAACCGCGACAA GTGGACTAAAGCGTCGGATT 45.0 50.0 58.1 58.0 482-491 bp 1.0 85.5
psbI-trnS-GCU_p5 TCTTGTACAACCGCGACAAA CAATTGGGAGGAGAGATGGC 45.0 55.0 58.1 58.0 504-513 bp 1.0 85.1