Hotspot

rps16-trnQ-UUG

LSC · 6480-7325 · 846 bp

Back to Geum

Pi 0.0297
MarkerSeek score 67.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
846
variable_sites
64
pi
0.0297
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CTCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.0 58.1 942-1014 bp 1.0 88.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 919-991 bp 1.0 87.6
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 930-1002 bp 1.0 87.0
rps16-trnQ-UUG_p4 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 940-1012 bp 1.0 85.3
rps16-trnQ-UUG_p5 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 941-1013 bp 1.0 85.2