Hotspot

rbcL

LSC · 54613-56046 · 1434 bp

Back to Gerbera

Pi 0.00628

MarkerSeek score 62.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 9
pi: 0.00628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTCATTGAA	50.0	45.0	57.1	58.0	1498-1523 bp	1.0	73.0
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTCATTGAAA	50.0	45.0	57.1	58.0	1497-1522 bp	1.0	72.9
rbcL_p3	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	45.0	45.0	57.0	58.0	1546-1571 bp	1.0	72.8
rbcL_p4	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.0	58.0	1545-1570 bp	1.0	72.7
rbcL_p5	TCATGAGTTGTAGGGAGGGA	ATTGGGCCGAGTTTCATTGA	50.0	45.0	57.1	57.8	1499-1524 bp	1.0	71.4