Hotspot

ndhD

SSC · 119938-121440 · 1503 bp

Back to Gerbera

Pi 0.002

MarkerSeek score 51.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGTTTCTCTGGTCCAAGTG	TCATTAGCCAAACAACTCTT	55.0	35.0	58.1	52.7	1589 bp	1.0	40.2
ndhD_p2	GGGTTTCTCTGGTCCAAGTG	TTCATTAGCCAAACAACTCT	55.0	35.0	58.1	52.7	1590 bp	1.0	40.2
ndhD_p3	GGGTTTCTCTGGTCCAAGTG	TTCATTAGCCAAACAACTCTT	55.0	33.3	58.1	53.6	1590 bp	1.0	40.2
ndhD_p4	GGGTTTCTCTGGTCCAAGTG	TTTCATTAGCCAAACAACTCT	55.0	33.3	58.1	53.6	1591 bp	1.0	40.2
ndhD_p5	GGGTTTCTCTGGTCCAAGTG	TTTCATTAGCCAAACAACTCTT	55.0	31.8	58.1	54.5	1591 bp	1.0	40.2