Hotspot

cemA

LSC · 60700-61389 · 690 bp

Back to Gerbera

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAGTCGACGAATGAGATGGG	GAATCTCCGGGATGGGTAGA	55.0	55.0	57.9	58.0	839 bp	1.0	79.0
cemA_p2	CGACGAATGAGATGGGTTCA	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.7	58.0	835 bp	1.0	77.7
cemA_p3	GTCGACGAATGAGATGGGTT	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.7	58.0	837 bp	1.0	77.6
cemA_p4	GAGTCGACGAATGAGATGGG	CTCCGGGATGGGTAGAAAGA	55.0	55.0	57.9	58.2	835 bp	1.0	77.4
cemA_p5	GAGTCGACGAATGAGATGGG	TCTCCGGGATGGGTAGAAAG	55.0	55.0	57.9	58.2	836 bp	1.0	77.4