Hotspot

psbE-petL

LSC · 64124-65412 · 1289 bp

Back to Gerbera

Pi 0.00233

MarkerSeek score 60.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 3
pi: 0.00233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.9	56.6	1435 bp	1.0	68.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1358 bp	1.0	67.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCCGTTAGTAGAAAACCGA	45.0	45.0	58.2	56.6	1444 bp	1.0	67.6
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	58.2	56.6	1449 bp	1.0	67.5
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1443 bp	1.0	67.4