Hotspot

accD-psaI

LSC · 58093-58765 · 673 bp

Back to Gerbera

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	854 bp	1.0	66.1
accD-psaI_p2	TCCAGAAGGGCTTATTCGATC	AAAGGCACCAAAAGAGAGGG	47.6	50.0	57.2	58.0	815 bp	1.0	66.1
accD-psaI_p3	TCCAGAAGGGCTTATTCGATC	GGCACCAAAAGAGAGGGAAA	47.6	50.0	57.2	58.0	812 bp	1.0	66.0
accD-psaI_p4	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	853 bp	1.0	66.0
accD-psaI_p5	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	855 bp	1.0	66.0