Hotspot

ndhC-trnV-UAC

LSC · 49717-50858 · 1142 bp

Back to Gerbera

Pi 0.00175
MarkerSeek score 59.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1142
variable_sites
2
pi
0.00175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCGGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1290-1292 bp 1.0 78.5
ndhC-trnV-UAC_p2 GGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1287-1289 bp 1.0 78.3
ndhC-trnV-UAC_p3 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1285-1287 bp 1.0 76.1
ndhC-trnV-UAC_p4 TTCGGGTCCTTTGCTAATCG GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1289-1291 bp 1.0 75.8
ndhC-trnV-UAC_p5 GGGTCCTTTGCTAATCGGAG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 1286-1288 bp 1.0 75.6