Hotspot

trnR-UCU-trnT-GGU

LSC · 29535-30662 · 1128 bp

Back to Gerbera

Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label trnR-UCU-trnT-GGU

Feature Metadata

label_name: trnR-UCU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 3
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnT-GGU_p1	AGAAGACCTCTGTCCTATCCA	GATTTGAACCGGGGGCTTAC	47.6	55.0	56.9	58.9	1224 bp	1.0	56.6
trnR-UCU-trnT-GGU_p2	AGGTTTAGAAGACCTCTGTCCT	GATTTGAACCGGGGGCTTAC	45.5	55.0	57.9	58.9	1230 bp	1.0	55.9
trnR-UCU-trnT-GGU_p3	AGAAGACCTCTGTCCTATCCA	AGCCCCTTATCGGATTTGAAC	47.6	47.6	56.9	58.1	1236 bp	1.0	55.2
trnR-UCU-trnT-GGU_p4	AGAAGACCTCTGTCCTATCCA	ACAAAGCCCCTTATCGGATT	47.6	45.0	56.9	56.9	1240 bp	1.0	54.8
trnR-UCU-trnT-GGU_p5	AGGTTTAGAAGACCTCTGTCCT	AGCCCCTTATCGGATTTGAAC	45.5	47.6	57.9	58.1	1242 bp	1.0	54.6