Hotspot

atpF-atpA

LSC · 27758-27815 · 58 bp

Back to Gerbera

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATATTGGCATATTGGGGGCG	ATTTCGTCGGCTTGAATGGT	50.0	45.0	58.1	58.2	125 bp	1.0	78.0
atpF-atpA_p2	ATATTGGCATATTGGGGGCG	GTCGGCTTGAATGGTTACCA	50.0	50.0	58.1	58.2	120 bp	1.0	78.0
atpF-atpA_p3	ATTGGGGGCGATGAAAGAAA	ATTTCGTCGGCTTGAATGGT	45.0	45.0	57.8	58.2	115 bp	1.0	77.4
atpF-atpA_p4	TTGGGGGCGATGAAAGAAAT	ATTTCGTCGGCTTGAATGGT	45.0	45.0	57.8	58.2	114 bp	1.0	77.4
atpF-atpA_p5	TTGGGGGCGATGAAAGAAAT	GTCGGCTTGAATGGTTACCA	45.0	50.0	57.8	58.2	109 bp	1.0	77.4